Dear all,
I want to inject (i.p) 10mg/kg of Fenretinide to mouse. First, I made a 25mg/ml stock in DMSO. Then I tried to reduce the final concentration of DMSO by diluting the stock to 1.25mg/ml using PBS. Unfortunately, fenretinide precipitates :(
Try to dissolve it in something else. We use olive oil as lipophilic vehicle...and in most cases it works perfectly.
Add drop wise into PBS with continuous sonication, may be possible.
I've found some related articles:
1.- "Single-dose Injections
C57Bl/6 mice received intraperitoneal injections of fenretinide [...]. Fenretinide was dissolved in dimethyl sulfoxide (DMSO) and resuspended in warmed PBS" 10.1074/jbc.M112.359950, which refers to PMID:15261805
"Control mice were similarly treated with i.p. injections of 5% ethanol in 0.9% sodium chloride solution containing 1.65 mg/ml of bovine serum albumin" 10.1371/journal.pone.0110410, which refers to 10.1016/S0959-8049(00)00335-X
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