How do I add an ascorbic acid solution to my cell culture media that I can ensure is sterile ?

01 November 2024 1 3K Report

Hi all,

I am working on creating my complete media for VSMC. I have to reconstitute 50mg of ascorbic acid in 10ml H2O (or base media) and then add 2.5ml into my final volume of 250ml complete culture media. How do I ensure the ascorbic acid is sterile when adding to my media after weighing out the product and dissolving the powder into solution?

Malcolm Nobre

Hello Ashley,

After having prepared 50mg of ascorbic acid in 10ml sterile H2O/ base media, filter sterilize the ascorbic acid solution using 0.22 μm syringe filter and then add 2.5ml of this sterile ascorbic acid solution to the final volume of 250 ml of complete culture media. This will ensure that the ascorbic acid is sterile when added to the media.

Best.

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