How can I make a preparation of 1M pH 6 potassium dihydrogen phosphate buffer?

I can't seem to find the answer online or in my lab anywhere.

09 February 2020 8,490 3 View

How do you lyse small cells (10 microns) without disrupting the mitochondria?

We consistently isolate intact/functional mitochondria from liver and heart tissue using manual homogenization (to disrupt the cell membrane) followed by differential centrifugation. I am now...

04 November 2019 9,279 1 View

In your research with OrganoTropisms are you utilizing tissue culture or histology slides?

Tropisms for various organ systems by borreliae have intrigued me for many years. In my research I have utilized autopsy tissues and tissue cultures for examination with Immunohistochemistry and...

17 March 2019 773 1 View

Why are we conscious?

07 August 2016 5,818 2 View

Should we have a basic income in the UK?

07 August 2016 1,723 0 View

What system mediates and incorporates all the potentially competing signalling of the brain?

Does the brain work in harmony, for example in a way where signals work up a chain of command and through the way the modularities of the connectome are integrated they are all given a proportion...

07 August 2016 4,341 0 View

The processing power of the brain, way-beyond binary; Could a Turing like machine decode the encryptions of the human brain?

We clearly process information, the neurochemical signatures are a way neuronal communication occurs between modulars of the brain. We have at least 50 neuro communication chemicals, that is...

07 August 2016 4,129 0 View

How can one make a dilution series before determining titer?

11 March 2015 7,875 3 View

Do you remove the Peyer's patches when doing FACS analysis on the mouse colon or include them in the analysis?

I am looking at the impact on the immune system with different treatments. Should I include the Peyer's patches when putting cells through flow cytometry? Or should they be removed and ran separately?

09 September 2014 8,874 5 View

A placebo-controlled investigation of synaesthesia- like experiences under LSD?

01 January 1970 8,512 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View