Recently, I want to use transwell assay to study glioma cells migration and invation. I have looked for a lot of protocols. And there were different opinions. I didn't find a complete and convenient protocol. What reagents and materials do I need to do this experiment? Thanks.
Dear Dr Wu,
Attached is the protocol we use. We get this from Corning.
Best Wishes!
Hi Shen Wu:
i totally understand what you are talking since i had similar experience several years ago. Quite a few candidate experiments suitable for this aim, such as transwell assay, invasion assay, and wound healing assay, every experiment has its cons and pros, and none of them is edge-cuttingly convincing. Since this work is critical for my project, i tried all 3 of them, the results generally consistent, so i drew a conclusion.
If this piece of work is not so sedimentary for your whole project, it would be a good idea to choose the one Charles recommended above.
Best regards
Shiqiu
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