Shiqiu Xiong

3 Questions 65 Answers 0 Followers

Questions related from Shiqiu Xiong

How to polarize human Th1/Th2/Th17 from bulk primary T cells?

Hi there: I am trying to establish a reliable and low cost protocols for human Th1, Th2 and Th17 polarization, does anyone has good experience to share? Many thanks for any information Shiqiu

07 October 2022 5,481 1 View

What's the the best method to kill PBMC for aqua live/dead marker staining?

Hi there: I am working on 10 colour FACS in PBMC. For compensation reason, i need to induced PBMC to die, then it can stained by LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit staining. To my...

15 April 2019 1,432 7 View

How to avoid working on dead cells?

Mammal cells have evolved a perplex system to keep a balance between proliferation and death. A bulk of cells normally consist of live and dead/dying cells. Because of high auto-fluorescence of...

01 January 1970 1,478 5 View