When one sterilizes media, how do its protein constituents remain intact? Microorganisms that were in the media are presumably destroyed by protein denaturation. Are full proteins present in the media, or just amino acids?
Normally, most media contains generally vitamins, salts, hydrolyates, nutrients, carbon and nitrogen sources like glucose and yeast or beef extract respectively (which breaks down to simpler compounds like amino acids and peptides on heating because they are mostly autolyzed.)
Just like @Amin contributed some has Aprotinin which helps if the yeast is not autolysed.
If there is protein in the medium it is subjected to heat denaturation.
I think that growth medium (excluding serum) does not contain full-sized proteins, only hydrolysates or, in the case of defined media, amino acids.
The media generally contain a mixture of amino acids, glucose, salts, vitamins, and other nutrients. in some cases the media contain proteins such as albumin, transferrin, and fibronectin which denature by heating. however, the companies in some cases add heat protective agents to media such as Aprotinin
Normally, most media contains generally vitamins, salts, hydrolyates, nutrients, carbon and nitrogen sources like glucose and yeast or beef extract respectively (which breaks down to simpler compounds like amino acids and peptides on heating because they are mostly autolyzed.)
Just like @Amin contributed some has Aprotinin which helps if the yeast is not autolysed.
A basal medium should be prepared and autoclaved. Then, all heat-sensitive compounds should be filter-sterilized and added to the cold autoclaved medium, in sterile conditions.
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