Ana M. Martins

3 Questions 5 Answers 0 Followers

Questions related from Ana M. Martins

Native PAGE followed by SDS Page - protocol?

Hi, all, I am trying to check if two proteins form a heterodimer and did native PAGE.. I cut the gel slice containing the band where the putative complex is and now want to do SDS-PAGE to resolve...

08 December 2017 844 4 View

What DNAse should I use as a flow cytometry buffer to reduce cell clumpiness?

Dear all, I searched several buffer recipes for flow cytometry, and I need to include DNase to reduce DNA-associated cell clumpiness. Some protocols mention DNAse II while others mention DNAse I....

13 October 2015 1,403 3 View

Can a SDS-PAGE gel be stored overnight before performing silver staining?

Hi, colleagues, can I leave a SDS-PAGE overnight after running? Should I leave it in fixation solution (50% methanol, 10% acetic acid) or shall I perform the fixation for 1 h and then leave it in...

16 November 2014 6,350 6 View