I am planning to use CNS-derived mouse catecholaminergic cell line CAD (Cath.a-differentiated) in order to investigate my protein of interest during neuronal differentiation process. So, I will need to generate CAD cell lines overexpressing the protein.
Does anyone have experience with stable or transient transfection of CAD cells? I am just wondering what transfection efficiency is for this cell line?
I would be grateful for your recommendations.