Each compound was treated in 1M HCl (dioxane–H2O, 1:1, 2 ml) at 100°C for 1.5 h. The reaction mixture was neutralized with silver carbonate and the solvent thoroughly driven out under N2 gas overnight. Furthermore, the residue in pyridine (1ml) was added to L-cysteine methyl ester hydrochloride (3.0 mg) in pyridine (0.1 ml) kept at 60°C for1.5 hr. Then butyl chloro tert dimethyl silane-trimethylchlorosilane (TCBDS-TMS) (0.6ml) was added to the reaction mixture and kept at again 60°C for 1.5hr. The supernatant (1.0 ml) was analyzed by GC/FI
I have no experience with TMSC (=TMCS?) as alone. I used 99% BSTFA + 1% TMCS). Maybe you look at this link? http://www.instrument.com.cn/Quotation/Manual/517262.pdf
Yes. You can use it. You can also use Acetic Anhydride/Pyridine. Try this...
Gud luck
:)
Why do you need to derivatize your phenol? If you're not getting good peak shape, then you probably have an injector problem, or you're using the wrong column.
Oh I'm living in iran and you know that provisions in my country is lows thus by this trend it's hard to work good in the labratory of university in iran. aboyt my research, the column is nonpolar than we need derivative phenolic compond. please help me about this subject and do you me what are you?
Ahh, understood. Yes, the acetic anhydride/pyridine derivatization is relatively easy - we've used it before.
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