suppose the solution is dilute. will there be risk of fluoride poisoning or phosphate burns if it comes into skin contact? the acetic acid would be 5-6% w/v, and then added excess Calcium carbonate to neutralize.
I am trying to pulldown my GFP fused protein of interest using ChromoTek GFP-Trap® Magnetic Agarose. My protein is not binding to the beads. Expression of the GFP-fusion protein has been...
02 June 2024 8,541 0 View
How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride and by which method and what solvent I should use ?
15 February 2024 4,834 0 View
How can I prepare 3 amino benzoyl chloride from 3 amino benzoic acid and thoinyl chloride by which method and what solvent should I use ?
15 February 2024 5,309 0 View
I'm getting the formate peak around 8.3 ppm. But the main problem is the quantification of the product. If you could give me some instructions on quantifying the product from the proton NMR, that...
07 February 2024 6,858 2 View
I need Global Car sales data (in units) from 1996 to 2021; especially Philippines and Indonesia. Kindly help, if possible send data or share the link. Thank You
14 December 2023 174 0 View
I lysed my intertidal phototrophic biofilm with hypotonic lysing solution containing 10 Mm Tris, pH 8.0, 1 Mm EDTA, 0.5% Triton-X 100, 1x protease inhibitor cocktail. after complete homogenization...
01 November 2023 5,759 0 View
What is the difference between ambient energy and renewable energy? Any reference material where I can study these energy sources?
03 September 2023 6,849 7 View
I'm working on a BET prototype but I'm facing problem in dead space.
28 June 2023 5,609 1 View
I have tried with ethidium bromide and stains-all for staining but not getting any band after one day of incubation. I tried with both Native and Non-Native gel.
05 June 2023 377 3 View
If we change different type of materials which are in the range of type 4 isotherm and they have similar pore size do we still need to tweak the dosing pressure for both the materials? Even if the...
22 May 2023 9,706 1 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...
01 August 2024 572 0 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?
28 July 2024 5,331 1 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...
21 July 2024 2,476 2 View
Chemical Engineering /Food Engineering
20 July 2024 4,152 4 View
My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...
18 July 2024 1,390 2 View
I'm trying to create a new substance. Are there any important considerations when fabricating with CVD? For example, lattice constant If you have any such considerations, please let me know.
15 July 2024 9,359 0 View