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Prevalence of HIV/AIDS as of 2025
24 September 2025 902 1 View
Fmoc-Cl+NHS gives Fmoc-Osu during this reaction which impurities formation ? I prepared it, obtained 99% HPLC purity. But 10% solution of Fmoc-Osu in acetone solution was not clear, hazyness was...
21 August 2025 9,111 0 View
tips on guidelines.
06 June 2025 6,545 2 View
I am trying to figure out if it is possible to run ddPCR reaction products on an E-Gel and actually get some distinct, visible bands. We think there may be multiple PCR products being generated...
10 April 2025 5,403 4 View
I am working on ddPCR assays to detect a particular gene. I can input a dilution series of gBlocks from about 10^5 copies down to 10 copies per reaction. This input can be determined based on...
15 February 2025 6,076 1 View
I am trying to amplify two DNA targets in a duplex PCR for diagnostic purposes. Targets A and B are normally amplified in singleplex PCR with their respective primers. Targets A and B are also...
04 February 2025 617 6 View
It has been found that the attitude of public sector employees is very impoverish and show less innovative behavior or agility then as compare to the private sector employees . What could be the...
04 February 2025 6,797 2 View
When I treat results using Processing Setup/Quan Browser I have results very different than I use Freestyle to obtein peak areas. I don't know wich result to trust. I'm using a LCHRMS.
14 December 2024 3,626 0 View
--Hi, I've calculated several LODs in qPCR (one LOD per probe) and I'd like to know if it's okay to average the LODs for my experiment? thank you --
11 December 2024 8,665 0 View
--Hi, is there a formula to compute the p-values from the ddPCR output ? I have an excel file outputed from Biorad QX600, i have a few columns with the CNV, PoissonCNVMax, PoissonCNVMax,...
28 November 2024 6,711 0 View
To begin with, lets recall: (6) Where might the new iron/ silicone/ lithium curtain be found? |...
23 November 2024 882 0 View
I ask because biomolecular water or TE doesn’t maintain DNA integrity as expected.
11 November 2024 6,613 3 View
I am interested in knowing if the CSR practices undertaken by Indian companies deal directly or indirectly with climate financing. Ideas?
09 November 2024 6,451 1 View
I have specific nucleotide sequence( from RNA-seq) and looking for a design assay enable me design primers that cover these specific nucleotides directly by forcing the primers to include them at...
06 November 2024 7,278 5 View
I am currently enrolled in PhD in Finance and my professional field is taxation in specific Sales tax/ VAT. As I have 8 years of experience as an VAT/Tax Officer in Pakistan, doing my thesis in...
06 November 2024 2,095 2 View
I am currently working with digital PCR for the detection of DNA and RNA pathogens in various pig tissues. However, I am experiencing an issue with RNA isolated from pig kidney tissue. I isolated...
28 October 2024 4,854 5 View
I'm working on Carbon Neutrality with CSR but i want to link with AI too. Is there anybody can help me with this. Is this a good idea for future research? If anybody give me some guidance it will...
24 October 2024 778 2 View
I want a huge raw dataset that I can process myself to conduct the research. Kindly help with the dataset.
08 October 2024 5,107 4 View
In some industries, authority provides lunch for their staffs and workers. The kitchen store of these industry regularly receive the supply of frozen chicken meat from meat processing company and...
30 September 2024 350 2 View
My academic pursuits align with my broader goal of connecting traditional cultural expressions with modern pedagogical methods, ensuring that future generations can experience and appreciate...
29 September 2024 8,289 3 View
I am trying to use PEG as a means of calculating the OSI of human milk after storing it at different temperatures (4C, -20C, -80C). Going forward, I will be testing a bunch of different donor...
27 September 2024 292 0 View
-Hi, is there some recommendations to represent digital PCR gene expression in barplot graph: Do i need to use ratio or concentration (copies/ul) ? thank you --
23 July 2024 2,192 1 View
I am working on a literature review of studies that systematically compare at least one case of collaborative governance to one or more cases of conventional governance (or that compare multiple...
26 June 2024 5,403 2 View
I have written a research paper all by myself with less than 5% Plagiarism and submitted it to my professor. But my professor is saying that my research work is AI-generated. How do I prove to him...
25 June 2024 9,463 4 View