I have labeled the specific neuronal protein through AAV injection and want to frequently observe the dynamic change of the same cell. I need go to the same spot every time. Could you please give me some idea about how to navigate to the same spot. Someones did suggest me to use the brain vasculature as a reference, but it needs to inject some fluorescent dye intravascularly which is not feasible for my project. I also am thinking about etching some labels on the cranial window with some fluorescent stuff, but I haven't figured out how to do. Could you please tell me something about how to observe the same spot using 2-photon scope, I will be very grateful!
Hi Jianjun Zhong ,
Good question! What brain region/cortical region are you working on? Is it possible to tag any landmarks (fibers...)?
If you cannot use vasculature as a reference for coordination, I guess there should be other cytoarchitectural differences along your ROI tissue or among different layers if it is cortical, such as the differential density of neurons (cell bodies or axons) in different layers or their perturbations. Neighboring nuclei, fibers or neuronal landmarks may also help.
Article Removable cranial windows for long-term imaging in awake mice
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