How could i get the Quantitative bone histomorphometric results?
- I've been puzzled about how to get the Quantitative bone histomorphometric results,such as number of osteoblasts (N.Ob),number of osteoblasts per bone perimeter (N.Ob/B. Pm) ( and osteoblast surface per bone surface (Ob.S/BS).
- Do you have any good suggestions ？Thanks
Dear Dr. Liu Weidong,
you could try the freeware:
ImageJ / Fiji
http://fiji.sc/Fiji
A plugin for bone can be downloaded here:
https://www.liverpool.ac.uk/ageing-and-chronicdisease/bone-hist.
It is for instance described in:
van ’t Hof, R.J., Rose, L., Bassonga, E., and Daroszewska, A. (2017). Open source software for semi-automated histomorphometry of bone resorption and formation parameters. Bone 99, 69–79. https://doi.org/10.1016/j.bone.2017.03.051.
A good start would probably be to familarize yourself with the nomenclature used in bone histomorphometry described in the following excellent article:
Dempster, D.W., Compston, J.E., Drezner, M.K., Glorieux, F.H., Kanis, J.A., Malluche, H., Meunier, P.J., Ott, S.M., Recker, R.R., and Parfitt, A.M. (2013). Standardized nomenclature, symbols, and units for bone histomorphometry: a 2012 update of the report of the ASBMR Histomorphometry Nomenclature Committee. J. Bone Miner. Res. 28, 2–17. https://doi.org/10.1002/jbmr.1805.
Good luck
Stefan Tangl
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