Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View

Has anyone tried performing a transient transfection on a cell line that has been stably infected with a lentivirus?

Hello, I was having difficulty in infecting a cell with a gene of interest. Say, for instance, I was able to introduce a CMV on the cell, but I was not successful in introducing my target gene. I...

25 September 2019 2,412 3 View

Mouse Cell Line STR Profiles?

Does anyone have STR profiles of mouse cell lines from the following strains: C57BL/6 129 FVBN BALB/c Swiss black Which company did you use to authenticate the cells?

07 April 2019 4,519 2 View

Can 2 way ANOVA be used in nonexperimantel research?

We will be studying about the role of spirituality and gender ( 2 IVs) on coping effectiveness (DV) and we shall be using standardized scales in measuring. Can 2 way ANOVA be used in our analysis?...

09 November 2017 8,917 4 View

Any information or studies about Wild Sampaguita (Jasminum bifarium) ?

My study for my undergraduate thesis is Phytochemical Evaluation, Antibacterial Activity and Angiogenic Property of Wild Sampaguita (Jasminum bifarium). I am using leaves for my study and I need...

25 March 2017 3,487 3 View

Qubit measurements of gDNA increasing over time?

Dear all, I am facing Subit issues, for mosquito genomic DNA measurements (for gDNA library preparation for NGS). The same sample was measured by 3 different people (in december 2015, august 2016,...

26 February 2017 2,726 5 View

Why don't we have bacteria growth in our liquid LB ?

We are facing a problem in different groups in our institute: when we grow E. coli (DH5a) overnight in liquid LB, we have: - no growth at all - sometimes, some tubes show cell growth, some...

20 February 2017 2,314 12 View

Does anyone have troubleshooting experience for low on-target exome enrichment (Nextera)?

I have prepared 4 libraries (Nextera exome 37mb), first with 12 samples and prepared 3 months before the next 3 (of 24 samples). The first had low enrichment (40% of reads on-target), the second...

30 June 2015 3,324 1 View

Can I use Agencourt Ampure XP for genomic DNA purification?

I have genomic DNA eluted in TE buffer, and am planning to sequence them. Unfortunately, EDTA should be avoided for Nextera library preparation. I am thus planning to purify my gDNA again, using...

19 March 2015 4,615 22 View

How common is five-fold symmetry among plants and animals?

06 July 2014 6,460 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

What influences inter-day reproducibility in UPLC-MS/MS analysis?

I was analyzing some metabolites in human plasma. While the intraday precision of these metabolites was acceptable ( less than 20%), the interday precision was quite large. So what influences...

13 February 2021 9,183 9 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

A good day to you, Great minds. How do I evaporate hexane and Ethyl acetate extract of bacterial metabolite?

I'm really struggling with it. It takes hours on a water bath at 50 degrees

03 February 2021 1,619 3 View

How can I measure speed of integration of an acquisition with secondary data?

I am currently faced with the challenge to measure integration speed of an acquisition or a merger with secondary data. Usually companies do not communicate once a target has been integrated...

27 January 2021 9,741 11 View

Can i re-incubate a WB membrane with a higher solution of the previously used 1ry ab?

I've incubated my western-blot membrane with both primary and secondary antibodies and i just realized at the end that i've used a lower concentration of my primary antibody for my loading control...

27 January 2021 1,034 3 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

Why some samples don't give any peaks in HPLC when the rest does?

Hello everyone, Last week we ran an HPLC assay with bacterial supernatant samples. The metabolites measured were SCFA and I used a dilution factor of 2 for some samples and 7 for others (because...

22 January 2021 8,667 6 View