WHOQOL - bref. test: how to conduct the correction?

18 January 2021 7,257 2 View

I need recommendations about where I can buy "ACRYLITE IR acrylic 11460" for use in zebrafish behaviour studies?

I need the "ACRYLITE IR acrylic 11460" for the light/dark preference test

16 November 2020 2,235 1 View

Is there a plausible explanation for the increased frequency of severe hurricanes in the southwest Atlantic Ocean other than climate change?

The Southeastern region of the United States of America, including Florida, Georgia, South Carolina, North Carolina, and the Caribbean archipelagos are being devastated by a higher number of...

16 November 2020 9,463 20 View

How can I differentiate the true allele from stutters and PCR artifacts in microsatellite genotyping?

Most of my microsatellite markers are dinucleotides, therefore they show stuttering. In regular stuttering is easy to identify the allele, which is the highest peak on the right, after the shorter...

26 September 2020 1,486 5 View

Only got one ligand pose after Glide docking?

I am doing docking with glide but the problem is I am not sure if the result I get is correct or not. After docking there is only one ligand pose is generated. Is it okay to have only 1 ligand...

02 August 2020 9,231 3 View

Can violent storms be caused by the moon in perigee?

It seems that the moon is getting farther away from Earth. Yet, storms are getting more violent. Why? Do these ferocious storms (tornadoes, hurricanes, rain-wind-thunder-lightning storms) occur...

25 December 2019 2,706 13 View

Problem with CaM antibody from Cell Signaling?

Hello I am currently trying to get a specific band for CaM (17kDa), according to Cell Signaling; the antibody which the lab bought, I should use: 1:1000 for primary dilution in 5% BSA in TBST all...

04 June 2019 4,151 3 View

How can an electron lose and gain mass?

I am wondering how an electron can gain and lose mass. What is the electron actually gaining and losing that causes its mass to change? Specifically, my application is in an electron beam...

07 May 2019 2,501 4 View

Can someone help with crash prediction models using GEE in SPSS?

I have volume and crash data for 7 years from several roadway segments. I used segment ID as my subject and Year as within subject variation. Then I used volume as predictor and total crashes as...

15 February 2019 3,129 2 View

How to release a protein (80 kDa) from a polymeric nanoparticle?

10 November 2018 6,963 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View