thickness of the slides and which protocol to follow
Once I tried to extract RNA from intestine of tiques (Amblyomma sculptum), I think it could be a little similar.
The tissues was eluted in PBS buffer and performed maceration until homogeneous.
The rest of the protocol I have and really old paper in my PC (attached)
I hope it was helpful.
Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...
17 February 2021 2,896 3 View
I found so many papers aplying the Deep Deterministic Policy Gradient (DDPG) algorithm implementing a critic neural network (NN) architecture where the action vector skips the first layer. That...
29 September 2020 2,572 2 View
Is there any dataset with heat transfer coefficients to train a neural network?
13 February 2019 3,235 2 View
I'm working in model consist of RC column subjected to lateral cyclic load in addition to constant axial load. in experimental paper rate of loading in lateral cyclic is 0.3 mm/second. and cyclic...
25 December 2018 4,632 3 View
I grow LNCaP prostate cancer cells in T flasks (vented neck, filter cap) in RPMI 1640 medium supplemented with 10% FBS and 1% Pen-Strep. While subculturing the cells, I am observing a strange...
04 March 2018 3,517 1 View
I am trying to check the in protein quality of a chickpea cultivar and for that we need to calculate the in vitro digestibility of the chickpea protein.I am following the protocol of Hsu.et al....
13 February 2017 6,596 5 View
We are analyzing the soil organic matter (SOM) in cultivated soils in the Provence area under Mediterranean climate (500-800 mm/year) with my students in agroecology. Soils in the area are usually...
01 January 1970 7,039 12 View
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...
02 March 2021 2,735 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...
01 March 2021 9,332 6 View
I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.
01 March 2021 4,306 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...
01 March 2021 8,079 2 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View