Skin Images differences algorithm ?

05 May 2019 5,664 3 View

Is there the possibility to detect a specific aminoacid sequence from proteomic data?

I am planning to LC/MS experiment to characterize the proteome of a specific biological fluid. I am mainly focused on detecting specific cleaved isoforms of my protein of interest. Is there any...

31 March 2019 9,846 3 View

What is the alternative for Illumina Tn5 transposase (ATAC-seq)?

Hi all, I recently found out that Illumina discontinued the DNA Nextera kit, replacing it with another one. As you might know, the enzyme from this kit (Tn5 transposase, TDE 15027865) is widely...

17 January 2019 8,066 48 View

Has anyone experience with polishing glass with a Struers Tegramin or similiar devices?

Which suspensions, lubricants and polishing cloths are usefull? How much time does it typically take to polish glass to get a roughness on the nanoscale?

10 January 2019 8,913 2 View

Transgenic reporter mouse line vs Immunostaining?

Dear scientific community, I faced an unexpected problem regarding the detection of a protein in the murine muscle stem cells. I am interested in a GPCR, which has a relatively low mRNA...

17 July 2018 7,299 4 View

Hi, If I want to measure cancer cell death after a protein-drug treatment which FACS protocol should i use? Annexin V staining ,PI staining or both?

Cancer cell line/Apoptosis/FACS/annexin V, PI/ drug

08 May 2015 2,327 7 View

H&E staining together with Immunofluorescence?

Dear Researchers, I have a batch of slides (mouse muscle cryosections) from a pretty precious origin, and I try to make the best use of every slide. I made a staining with an antibody (Alexa...

01 January 1970 1,167 7 View

Do you know that Chaos is complete increased Entropy which can not exist in living organisms of whole living Nature?

01 January 1970 2,258 14 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View