Lay the filter on top of a Petri dish with nutrient agar appropriate for the bacteria you want to detect, incubate the plate at the proper temperature. The medium will diffuse through the filter and feed the bacteria collected on the filter, generating colonies that you can count. I assume that you can then calculate the n° of colonies per 100 ml.
you can prepare serial dilution of the 100 ml solution and then plate each dilution onto the appropriate culture media, and keep in the incubator. Allow the colonies to form. Then you can calculate no. of colonies using the CFU/mL
CFU/mL = No. of colonies * dilution factor / volume of culture plated (ml)
If you filtrate 500 mL in one membrane, the amount of growing colonies corresponds to # CFU/ 500 mL. If you want to report in 100 mL, you must divide the #CFU / 5 to obtain the concentration in 100 mL!
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