How could I analyse rt-qPCR expression in between untreated and treated Tyrosin Kinase Inhibitor in chronic myeloid leukemia BCR-ABL1 gene Expression?

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Can any one send me some articles related to the role of MPS1 kinase in CHK2 actvation?

07 August 2016 4,368 0 View

What is appropriate incubation time for primary antibody in western blot analysis?

Primary antibodies, Incubation time, western blotting, ELISA, SDS-PAGE

07 August 2016 6,517 22 View

For phosphospecific kinase proetin how much time incubation (45') for secondary antibody is good for perfect result and washing time after incubation?

western BLOTTING, SECONDARY ANTIBODIES, PBS, Incubation time, SDS-PAGE

06 July 2016 9,545 2 View

Can blotting effect if some amount of SDS-Buffer present in transfer buffer solution?

06 July 2016 1,066 5 View

I am using BSA for blocking membrane for Phosphospecific antibodies but getting much background, could any one help me how to overcome this problem?

06 July 2016 2,109 4 View

I am working on CHK2 kinase protein and every times after western blot getting blackground stain also, washed 3 times10 minutes each,what is problem ?

western blotting, SDS-PAGE, CHK2, CHK1, Washing buffer, PBS, TBS

06 July 2016 9,108 8 View

For phosphospecific antibodies why milk blocking buffer is not good?

06 July 2016 5,507 1 View

Effect of ethanol on MCF-7 breast cancer cell line?

03 April 2016 5,196 2 View

Can any one please send me rad 9b gene related research article??

02 March 2016 3,858 0 View

What is main difference between Ct and Cq value? When do we use Ct value and when Cq In Real Time PCR?

11 December 2015 612 13 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

No title

02 March 2021 3,060 3 View

Divaker Choubey

What seems to be the problem? Are you not getting any amplification or what? Without specific problem identified, it is impossible to address the question concerning qPCR. Several steps need to work to get an interpretable observation.

Yogendra Verma

I am confused in statistical analysis of the data (SEM and p value) in both untreated and treated sample, how to compare these data are up-regulated or down regulated???, and in interpretation of results of these sample.??