I am studying effect of cigarette smoke on Airway epithelial cells. I am confuse regarding cigarette smoke induced cell death in cells. some papers says that CS causes necrosis and little apoptosis. some says, Cs can induce apoptosis, some says at lower concentrations it can also induce proliferation.
Please guide me .
CS, depending upon the dose and the contents of CS, in certain cell-types induces cell growth arrest or cell death. Cell death could be due to necrosis, apoptosis, or pyroptosis. Therefore, it may be critical to optimize conditions for your experiments using your set-up to expose cells to CS and cell-type.
Hi Hina,
In order to evaluate how cigarette smoke induces death in cells, it’s important to accept that cigarette smoke (CS) is not a cyanide, and it does exhibit prolong effects as being the major cause of lung cancer. So, the best ways to anticipate CS acute and short-tern effects on airway epithelial cells would initially be to define how cigarette smoking alters the airway transcriptome genes, i.e., those that are normally expressed in a subset of human airway epithelial cells obtained at bronchoscopy, and details the cigarette smoke-induced changes in gene expression. The use of gene expression profiling to explore the normal biology of a specific subset of cells within a complex organ across a broad spectrum of healthy individuals and to define the reversible and irreversible genetic effects of cigarette smoke on human airway epithelial cells. Among the above genes you’ll find those susceptible to low doses of CS, and determine would they link to apoptosis or rather to proliferation pathways.
Best wishes,
Ilya
Direct effect of cigar on cell death. There are so many toxic compound which may cause it. Sure we can detect alteration of gene expression in cronic smokers. We should have in mind that toxic gas such as carbon dioxide, carbon monox may cross the membrane due to neutral charge. Also free radicals, toxic metabolic is produced. After the cell death the organism try to adapt and create resistant layer of epitelial cells.
Dear Hina,
I agree with the previous comment and its depend on nicotine concentration and cell type. a few years ago we published som data which could give you some answers and information about which genes that affected by nicotine or combination with alcohol.
Here below is our article.
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