Does anyone have matlab code how to use k-means clustering using feature extraction as an input, please ? and also how can we get the c1, c2, ..,cN?

hi, i'm really new to matlab and k-means clustering. i'm sorry for my question but i really get lost now. if anyone can help me or have matlab code how to use k-mans clustering using feature...

09 October 2018 2,954 8 View

Why using beads to measure phagocytosis assay?

I am doing phagocytosis assay to measure phagocytosis in Plasmodium infected RBC. This field is new for me, basically, I don't really understand why it is needed to do phagocytosis assay with...

07 February 2018 4,150 3 View

Is there a role for cultural activism in challenging orthodox research methodologies ?

Black Emancipatory Action Research includes at its core participatory and action research methodology which imperatively rely on community participation and community empowerment at all stages of...

23 June 2017 2,775 8 View

How to interpret the CV curve of cathode for Li-ion battery?

16 June 2016 5,003 3 View

Has anyone analyzed the severity of duty cycle in accerlated life testing for automoblie durability qualification?

11 November 2015 2,485 3 View

Can anyone give me some informations about the cyclic voltammetry of LiPF6 electrolyte?

Dear colleagues, I'm currently doing a research about Li-ion battery. In this case, I used LiFePO4 as a cathode. I got a peak of reduction around 0.8 V. I supposed that the cathodic peak seen...

12 May 2015 2,686 1 View

How can I synthesize ZSM-5 Free Structure Directing Agent by using hydrothermal technique?

I have synthesized some compounds by different variations of Si/Al, OH/Si, H2O/Si, temperature, condition (tumbling and non-tumbling) but I got almost the type of mordenite. There is also ZSM-5...

03 March 2015 6,411 6 View

Can anyone explain how to read the diagram of cyclic voltammogram?

I have diagram of cyclic voltammogram, seeking to understand how to define the diagram voltammogram.

06 May 2014 8,990 10 View

how can the voltage range be determined in aqueous electrolyte (Li2SO4) with LFP cathode and LTP anode materials?

Aquaeous electrolyte for lithium ion batteries

31 March 2014 5,218 1 View

How can I calculate mass of carbon in LiFePo4/C?

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15 February 2014 4,584 7 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View