How cell wall peroperties of l.lactis may change after surface display of a heterogenous antigen?

Hi there! We are applying L.lactis for expression of a heterogeneous antigen using a cell wall anchor subunit. Cloning process has been verified and expression optimization is up now but I'm...

07 August 2019 6,020 2 View

How can I determine the intact bacterial cell in flow cytometry?

Hi guys! I'm working on a whole cell SELEX project of bacteria for developing a diagnostic tool. Most of bacteria are less than 2 micrometer in size that is interfered with many noises . Please...

02 March 2018 8,100 5 View

Why FITC-nucleotide conjugate emission vanish during SELELX process?

Hi there! I'm using FITC-primer conjugate for amplification of DNA library. The florescent tag is necessary for investigation of SELEX proceeding by flow cytometry technique. The problem is...

01 February 2018 1,691 9 View

Am I allowed to run HCD test with Real-time PCR primers?

31 December 2016 3,358 0 View

How can I run PAGE for alkaline peptides?

31 December 2016 4,284 2 View

How can i install DSSP for gromacs on centos6?

Hi everybody I tried to execute dssp for gromacs 4.5.7 by following this instruction: #sudo wget ftp://ftp.cmbi.ru.nl/pub/software/dssp/dssp-2.0.4-linux-amd64 -O /usr/local/bin/dssp #sudo chmod...

03 April 2015 4,591 1 View

How long is an appropriate time for structural change investigation by MD?

Hey there! I'm using gromacs software for ligand binding simulation. I want to investigate structural change of protein after complex formation. How long the MD should last to reach the most...

02 March 2015 1,685 8 View

Why the h-bond may vanished during ligand binding simulation?

Hi there! I have performed MD simulation for protein-ligand binding. I used g_hbond command for protein and ligand interaction analysis. I observed that h-bond numbers reached from 4 to 0 after...

01 February 2015 8,191 8 View

Why is there a disparity between c-alpha elements and amino acid content?

Hi every body I was going to calculate RMSF for residues of protein after simulation. I chose c-alpha group which contained 667 elements. My protein has 679 amino acids and 50 of them are Gly. So...

01 February 2015 1,289 9 View

Any advice on binding energy calculation by Gromacs MD?

01 February 2015 1,137 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View