Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

What is the system of units used in the thermal conductivity by Spitzer and Harm?

23 February 2021 9,253 1 View

How many days do we need to detect adoptively transferred Treg in the tumor site in tumor-bearing recipient mice?

Hello, I've got a plan to isolate mouse splenic Treg (WT and KO of target gene) and inject these CD45.2 Treg into tumor-bearing CD45.1 mice. My question is how many days do I need to wait to see...

11 February 2021 8,238 1 View

Is it possible to antibody labeling with FITC cause antibody disfuction?

Hello, I studied how much bound my test antibodies to the target antigen using FITC labeling to test antibodies. I tested 3 types of antibodies, the elisa binding affinity and cytotoxicity...

07 February 2021 8,335 2 View

Can you use MTS assay with cells cultured in media containing phenol red?

I am trying to run a cell viability assay with LN229, but these cells are cultured in DMEM which contain phenol red. Is the MTS assay sensitive to the additional of phenol red in the media? If so,...

03 February 2021 9,478 2 View

Lighting for open field test behavior assay?

I am currently setting up our lab's behavioral space to run the open field test in rats, and I am trying to figure out how to set up lighting to evenly illuminate the open field chamber (a 90 cm x...

26 January 2021 1,399 3 View

Is there a surface inactive water-soluble dye?

Hi guys, I'm looking for a water-soluble dye which is not surface inactive in order to observe (water in oil) emulsions; I don't prefer to use oil-soluble dyes. I've used rhodamin123,...

21 January 2021 8,496 2 View

Can I calculate the average from the data which are collected every 30 minutes for ANOVA?

I artifically elevated the air temperature in the heated plot by 3-celsius degree compared to that in the control for 7 days. I want to figure out the effect of the temperature manipulation on...

21 January 2021 1,185 8 View

Inaccurate molecular weight in SDS-PAGE?

I ordered a synthetic peptide (sequence = MHHHHHH) This peptide has a molecular weight of 0.97 kDa based on a web-based prediction tool and mass spectrometry. However, when I ran it on the...

20 January 2021 3,439 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

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03 March 2021 5,360 2 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

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01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View