Yesterday- where, when, with whom ?

what are the next steps? kindly see the attach

01 January 1970 1,014 0 View

Can a scientist put the right questins and give wrong ansewrs?

or are the errors responsabilizing the whole community? a linkincluding Nobel prizes http://ciret-transdisciplinarity.org/index_en.php 3 surprising attached files

01 January 1970 8,348 0 View

Quantum computing - which model application?

according to http://cfcul.fc.ul.pt/equipa/apato.php 2 thesis (Master and forthcoming PhD) Bohr and De Broglie are 2 extremes of quantum theories. the advent of quantum in computing hardware, as of...

01 January 1970 4,139 0 View

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01 January 1970 3,153 2 View

Have you read on Antonio Giao ands his vast work?

01 January 1970 5,794 0 View

Do you enjoy reading alphabetic library goo.gl/ZuJKaD ? how many books?

what to do with so many interesting texts ?

01 January 1970 3,230 0 View

Do you beleive in the truth of these literary stories?

with 1 exception, inspired by a Hitchcock movie, all of them happened in Asia, Africa, Latin America consider their translation

01 January 1970 9,238 1 View

A new template for curriculum vitae, how do you see it?

planning to rewrite my CV in this model- the same i use for biography studies

01 January 1970 3,161 2 View

May i submit papers and links?

for instance i coordinated emmasia.uevora.pt plus emmasia.udn.vn and made 3 reports plus 1 draft are these relevant?

01 January 1970 9,546 0 View

Why is it that India reads more than rest of the world?

see this https://goo.gl/#analytics/goo.gl/ZuJKaD/all_time

01 January 1970 4,416 1 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

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22 February 2021 9,932 11 View