Yesterday- where, when, with whom ?

what are the next steps? kindly see the attach

01 January 1970 1,014 0 View

Can a scientist put the right questins and give wrong ansewrs?

or are the errors responsabilizing the whole community? a linkincluding Nobel prizes http://ciret-transdisciplinarity.org/index_en.php 3 surprising attached files

01 January 1970 8,348 0 View

Quantum computing - which model application?

according to http://cfcul.fc.ul.pt/equipa/apato.php 2 thesis (Master and forthcoming PhD) Bohr and De Broglie are 2 extremes of quantum theories. the advent of quantum in computing hardware, as of...

01 January 1970 4,139 0 View

How can you collaborate in the new project we are building?

Contribution within Asia New ideas to implement Relation to local cultures Mutual understanding Creation of elibrary

01 January 1970 8,750 0 View

No title

01 January 1970 3,153 2 View

Do you enjoy reading alphabetic library goo.gl/ZuJKaD ? how many books?

what to do with so many interesting texts ?

01 January 1970 3,230 0 View

Do you beleive in the truth of these literary stories?

with 1 exception, inspired by a Hitchcock movie, all of them happened in Asia, Africa, Latin America consider their translation

01 January 1970 9,238 1 View

A new template for curriculum vitae, how do you see it?

planning to rewrite my CV in this model- the same i use for biography studies

01 January 1970 3,161 2 View

May i submit papers and links?

for instance i coordinated emmasia.uevora.pt plus emmasia.udn.vn and made 3 reports plus 1 draft are these relevant?

01 January 1970 9,546 0 View

Why is it that India reads more than rest of the world?

see this https://goo.gl/#analytics/goo.gl/ZuJKaD/all_time

01 January 1970 4,416 1 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

How to determine the significance of "diagonal" contrasts in two-way ANOVA interactions on SPSS?

Hi all, I'm running a 2 x 2 between-subjects ANOVA with the setup in the attached image (kindly refer to JPG attachment). I understand that calculating simple effects involves determining...

09 February 2021 547 3 View

During an anti-His capture ELISA has anyone had issues with histidine residues from secondary antibodies non-specifically binding?

Hi, Hope you can help. Currently been running some sandwich ELISA assays where I coat with anti-His, block and then capture His-tag protein and then sera and then anti-bovine monoclonal...

07 February 2021 7,101 1 View

How to clean up an already pure protein from grwoth medium?

Hi all, I have got a protein, expressed in P. pastoris vector, more than 97% (SDS-page) pure in the culture medium after cells separation. Despite the protein expressed has a his-tag, Nichel...

04 February 2021 5,173 6 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View