To distinguish between intact and pseudogenes is not a trivial task. There is a tool available at NCBI (http://www.ncbi.nlm.nih.gov/genomes/frameshifts/frameshifts.cgi) that uses a simple approach: It takes neighboring pairs of proteins and
does a BLASTP analysis on them and notes which neighbors hit the same longer
protein. Pairs that hit the same longer protein may represent a single gene that has gained frameshift or other mutations.
That is far from perfect, but works quite well for bacteria...
i suggest you to get a course on molecular biology (or take alook at book sur as Genes ) but roughly in few words coding sequneces are exons and non coding sequence are introns
if you look at your gene of interest in ncbi you can get the sequence and get details on where are introns and exons
I agree with Akbar Dorgalaleh. thanks a lot for your comment and answer.
Artur Burzynski dont forget here is RG and we should Respect for other inquiries.
You dont have to answer the questions.
Thanks dear Reyhaneh ,Akbar and Frédérique
Mr Artur Burzynski ·I respect your comment,too and because of this I prefer not to continue speaking and even supporting my own right here.
Mr Dorgalaleh and Mr hilliou I think because of my inappropriate decleration you replyed me another thing .My mean was about approaches which can help us to distinguish coding sequences and sequences like psedogenes which has same structure of codig ones.This question is one of our best professor"s and I'm sure that it's my fault .
Dear Artur I appoligize for taking your time because of reading this question and I respect you.No problem
Dear Atefeh see Pseudogene From Wikipedia (part of "Properties" describe in detail).
good luck
To distinguish between intact and pseudogenes is not a trivial task. There is a tool available at NCBI (http://www.ncbi.nlm.nih.gov/genomes/frameshifts/frameshifts.cgi) that uses a simple approach: It takes neighboring pairs of proteins and
does a BLASTP analysis on them and notes which neighbors hit the same longer
protein. Pairs that hit the same longer protein may represent a single gene that has gained frameshift or other mutations.
That is far from perfect, but works quite well for bacteria...
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