dear Dr. Prakash,

Can you be more specific about the composition of the gel, the % crosslinker - content of the acrylamide solution, and in particular, which buffering system you are using?

Conventional Tris-glycine gels, even 15-20% will only resolve down to around 10kDa ; everything below this will not be separated - hence the smearing you noted.

For proteins in that size range (

dear Dr. Prakash,

Can you be more specific about the composition of the gel, the % crosslinker - content of the acrylamide solution, and in particular, which buffering system you are using?

Conventional Tris-glycine gels, even 15-20% will only resolve down to around 10kDa ; everything below this will not be separated - hence the smearing you noted.

For proteins in that size range (

Dear Dr. Prakash,

I recommend you to use Tricine-SDS-PAGE.

Please try to make separation gel, spacer gel, and concentration gel for good separation.

Good Luck.

Kazumi Hiraga

Thank you sir. I will try doing so. After that, I will be coming back to you if any problem will come forth.