How can we partially reduce antibody by TCEP( TRIS 2CORBOXY ETHYL PHOSPHINE)?

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Kirstin A Zettlitz

We use 2.75-fold molar excess of TCEP and incubate for 1 hour at room temperature for partial reduction of Antibody IgG (1-10 mg/mL in PBS)

Sun MM, Beam KS, Cerveny CG, Hamblett KJ, Blackmore RS, Torgov MY, et al. Reduction-alkylation strategies for the modification of specific monoclonal antibody disulfides. Bioconjug Chem. 2005;16(5):1282-90.

Alok Shukla

Kirstin A Zettlitz thank you for your valuable answer