How can we determine that which method should be followed for conjugation of a toxin to proteins?

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Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

What is recommended for solid domain in ANSYS FLUENT?

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01 March 2021 9,785 3 View

Can Virtual Servers (remote dekstop manager) run heavy ANSYS Fluent CFD simulation ?

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The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How to include original NMR data in a manuscript?

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27 February 2021 734 4 View

Does different dilution factors affect negatively on NH3 reading ?

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27 February 2021 8,483 3 View

Can anyone share his experience regards Smoothing in origin?

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26 February 2021 8,183 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

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02 March 2021 8,437 2 View

How to simultaneously perform multiple biochemical assays?

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01 March 2021 6,999 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

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01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

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28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

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How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

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28 February 2021 3,951 2 View

Benefits of the 24 welled plate?

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28 February 2021 1,149 1 View

Edward Michelini

Pierce (now Thermo) has a huge array of reactive linking agents, usually with amine, thiol, carboxy or glycol reactivity and an array of spacer arm lengths, often including hydrophilic PEG. Its just a matter of finding the conjugate combination which works best for you. The book "Bioconjugate Techniques", 3rd Edition (2013) by Greg T. Hermanson is a good place to start, but Thermo has quite useful WEB pages as well.

Lisandra Herrera Belén

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Muhammad Naeem Iqbal

Bundle of thanks dear