Lisandra Herrera Belén

2 Questions 3 Answers 0 Followers

Questions related from Lisandra Herrera Belén

How can I remove C-terminal His tag from pET22b express protein and thrombin from N-terminal?

In designing a new chimeric protein and I will use pET 22b for it. But I have to include C-terminal His-tag and N-terminal thrombin. So I need to remove it after purification

16 August 2018 6,318 2 View

How I can determine the content of antibody bound to nanoparticles coated with polymers considering that these are not soluble in water ?

i´am working with iron nanoparticles functionalized with antibodies, and i need determine the ratio of antibodies on the nanoparticles.

11 June 2015 3,669 3 View