How to find the air side pressure drop in a spiral fin tube bundle cross flow heat exchanger?

How to find the air side pressure drop in a spiral fin cross flow heat exchanger? The pressure difference at the inlet and outlet is very low compared to the experimental results, which I...

18 February 2021 1,109 2 View

Which sources you would suggest for finding unpublished research articles in order to include in meta-analysis?

Which sources you would suggest for finding any sort of unpublished research articles (e.g., pre-prints, rejected manuscripts, articles from grey-area, dissertations) in order to include in a...

09 January 2021 7,269 7 View

What is the reason for observing two bands of beta-actin after lipofectamine transfection of keratinocytes cells?

25 December 2020 8,668 3 View

Does anybody knows anything about INFORMATICA JOURNAL (SCI expanded), which publishe article at a cost of 423 US/or equivalent bitcoin dollars?

Does anybody knows anything about INFORMATICA JOURNAL (SCI expanded), which publishes an article at a cost of 423 US/or equivalent bitcoin? Is this journal fake or true?

15 December 2020 8,985 2 View

What kind of a relation is there between the cement hydration and the concrete properties?

How are the concrete properties changed by the differences in hydration?

24 May 2020 4,009 10 View

Blood or tissue sample for cytokine assay?

Hello all, I would like ask whether blood or colon tissue could be a better option to analyze TNF-alpha, IL-1B, IL-10, IL-6 levels in mice colitis model. Also which method would be a better...

08 December 2019 5,442 3 View

Can you share the names of research centers which gather the video-recordings of adult psychotherapy sessions?

Dear colleagues, I would like to conduct my independent research on psychotherapy processes of individuals with pathological narcissism. I would really appreciate if you can share your contacts...

10 November 2019 6,193 2 View

Does Kapa genotyping kit suitable for TA Cloning ?

Hello, I am extracting mouse DNAs and performing PCRs through KAPA 2G Fast hotstart genotyping kit. Before I use the pcr products for TA Cloning (invitrogen), I am purifying PCR products through...

14 October 2019 2,984 2 View

I cannot detect Parp protein after total protein isolation?

I cannot detect Parp protein after total protein isolation Hello everyone; I cannot detect the Parp protein after total protein isolation. I can see my cells die morphologically after a novel...

18 July 2019 2,593 2 View

At what stage should I use the DNAse I reaction protocol?

Protocol content (NEB DNAse I reaction Protocol) : RNA: 10ug DNAse reaction buffer 1X 10ul DNAse (Rnase free) 1ul Nuclease free water 100 ul My question is; if I use this protocol after isolating...

11 January 2019 1,249 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View