I have calculated the values of Neoxanthin, violaxanthin, lutein, zeaxanthin, antheraxanthin and beta carotene and Chl a and Chl b by HPLC. How Can I calculate carotenoid content, total chlorophyll content and different ratios among these pigments?
What do you mean by values? Concentrations in your prepared solution of extract that you injected into HPLC? Calculate the total amount of each carotenoid, considering concentration, dilution factor, total volume of extract, and weight of plant material into equation. This is assuming you extracted all carotenoids (some are colorless - phytoene and phytofluene). You may then add up all carotenoid values (mg/kg dry weight or wet weight) to get total carotenoid content. Rough estimate does not require HPLC, only absorption of extract at ~450nm.