What type of PC calibrator should be used and does the Cutoff determination should be based on Positive control calibrator?

I'm trying to develop a chemi-luminescence based immunoassay for HIV antibody detection. For the purpose I'm using negative as well as positive control as a quality control. Currently, I'm using...

11 December 2019 3,066 2 View

How can the stability of Biotin conjugated/ HRP conjugated antibodies and antigen in ELISA/ Chemiluminescence assay can be achieved?

I'm trying to standardize a buffer in which my biotin conjugated and HRP conjugated molecules (antibodies and antigen) stay stable for longer time. My assay for detection of HIV works well with no...

05 June 2019 7,159 3 View

What should I use for cDNA synthesis?

I want to do cDNA synthesis and would like your suggestion regarding what to use, Oligo dT or Random primer. I would like if you also give me the reference for the same.

10 November 2012 9,768 25 View

How to determine the stability of Phosphate buffered saline (PBS)?

PBS is commonly used for many purposed in the labs, most of the time it is freshly prepared but what in case it is made and kept for longer time. How long can it be stored and how to access its...

01 January 1970 9,488 4 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View