Haemolymph should be collected in ice cold eppendorf tubes provided with sterile ice cold anticoagulant buffer (AC buffer; 0.098 M NaOH; 0.180
M NaCl; 0.017 M EDTA (free acid); 0.041 M citric acid; 440–450 mOs/kg; pH 4.8) (Xia et al 2000; Acid phosphatases in the haemolymph of the desert locust, Schistocerca gregaria, infected with the entomopathogenic fungus Metarhizium anisopliae. Journal of Insect Physiology 46 (2000) 1249–1257)