On the basis of SNPs I predict primers with the help of PrimePro software, how can I reduce the number of primers if I have a facility to order only 100 primers for PCR.
Using PrimerPro, you should get an output file called "[name] .fasta.results.csv". This result file contains the orignal misa output as up to 3 of the best primer pairs found by Primer3 along with their properties.
So this file should contain the 3 best primer pairs found. The first pair should also be the best pair. Which file are you looking at and how many primers are there?