How can I select the best plasmid after sequencing?

I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...

06 February 2021 336 3 View

What can be the problem in the isolation of Plasmid?

I've tried transformation of a 1.7 kb gene with DH5 alpha strain of competent cells and also used PuC19 as a control. After selecting colonies and broth-culturing overnight, I isolated plasmid....

27 December 2020 3,596 5 View

MiRNA seq differential analysis?

Hello, I am writing to ask your help in regard to performing differential analysis to analyze miR RNA-seq data. I have miRNA seq data and I have analyzed in the following way- Initial QC - removed...

19 August 2020 5,105 7 View

How can I know the rank of each node in RPL in NetSim after simulation?

I am able to successfully run IOT RPL simulations in NetSim but in the results window, animator and plots I am not able to find the rank of the nodes. Please let me know how can I find this. Thanks

03 August 2020 3,630 3 View

How to calculate RECEIVER OPERATING CHARACTERISTICS(ROC) curves and area under the curve (AUC) of microRNA data?

Hello, I am having micro RNA data sets of control and patients now I am interested into analyzing micro RNA as a biomarker for a particular disease. I have already delta delta CT values for...

30 June 2020 7,858 2 View

I can't run Mendeley desktop on Microsoft Word 2019 MSO. Does anyone have an idea?

I have added plug-in but it does not work. it shows error 429

04 May 2020 7,810 2 View

How can i reduce primer dimers and get my cDNA PCR amplification done?

24 March 2020 1,525 3 View

I need to do Measurement of spectral emissivity by a FTIR spectrometer?

Please let me knw where i can complete this test in india

06 February 2019 6,227 2 View

How to apply statistical model in scenarios where dependent/response variable is not defined/labeled?

I have a scenario where many people are using a software and while utilizing they are assigned values between 0 to 1 based on their good or bad usage. e.g. good use would provide value to "usage"...

26 March 2018 300 4 View

What are the common steps involved in text analytics projects?

If i have to get the most possible generic steps of text analytics, what are the most commonly used steps for any text analysis model. Any help and your expert guidance/ suggestions are...

29 August 2017 9,968 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View