How can one model/code for Momentum Capture in ABAQUS to capture waveforms at particular time points?

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Protocol for Inducing Necroptosis in Cell Culture ?

Hi I am looking for a protocol (with appropriate dilutions, concentrations, quantities) of inducing necroptosis in in vitro cell culture. I have found protocols using TNF alpha dosing and TAK1...

31 December 2019 5,895 3 View

Anyone had any issues with fibrin gels disintegrating in OCT for immunostaining?

I use oCT to put my gels into in order to under go immunostaining. It appears the gels disintegrate both the longer they are in OCT and when they are frozen, leaving wafer thin samples. Does...

05 June 2016 3,255 0 View

Has anyone got an experience in using Bovine Thrombin from sigma?

- particularly this one http://www.sigmaaldrich.com/catalog/product/sigma/srp6556?lang=en®ion=US. Can it be used for cell culture? Does it need purifying before use?

04 May 2016 1,826 2 View

Problems with Bovine Fibrin Clots?

Hi I am using Fibrinogen (10mg/ml) and Thrombin (1000U/ml) to form fibrin clots. I found that after getting good cross linking initially the clots dissolve to liquid if left in incubator...

04 May 2016 7,192 5 View

Anyone have a good methodology for Agar Gel for adding on top of a cell culture dish (and that allows you to pour/pipette as a thin layer)?

For Cell culture/bioreactor. Needs to be relatively quick setting

01 February 2016 9,357 1 View

For Fibrin Matrices is >2000 NIH/ml best - or can one use 600-2000NIH/ml in order to produce 1000U/ml concentration (Thrombin from Bovine Plasma)?

- used for making matrices to seed neonatal fibroblasts.

01 February 2016 4,234 1 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out?

Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out? For example consider a process for certain product manufacture where a new process flow...

31 July 2024 781 1 View

Help on understanding the implementation of Mori Tanaka method on MATLAB?

I am new to Micromechanics and having similar problem with understanding the implementation of the formula's. I would appreciate if anyone can guide me on how to go about getting a scalar value...

30 July 2024 969 0 View

Simulation of metal drawing by Abaqus with UMAT?

Hello, colleagues. Recently, I have been working on a metal processing simulation with my UMAT in Abaqus. I have outlined the corresponding simulation, but I keep encountering issues that cause...

30 July 2024 7,062 1 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View