- used for making matrices to seed neonatal fibroblasts.
Hi Rob, are you looking for cell adhesion to immobilized fibrinogen treated with thrombin. Or are you using soluble fibrinogen treated with thrombin and then allowed to immobilize onto plates?
Hi I am looking for a protocol (with appropriate dilutions, concentrations, quantities) of inducing necroptosis in in vitro cell culture. I have found protocols using TNF alpha dosing and TAK1...
31 December 2019 5,895 3 View
I am trying to model a waveform through different bars in Abaqus to mimic a SPHB. I wanted to add momentum capture so I can get values of the waveform at different time points. How does one do This?
03 April 2019 8,650 2 View
I use oCT to put my gels into in order to under go immunostaining. It appears the gels disintegrate both the longer they are in OCT and when they are frozen, leaving wafer thin samples. Does...
05 June 2016 3,255 0 View
- particularly this one http://www.sigmaaldrich.com/catalog/product/sigma/srp6556?lang=en®ion=US. Can it be used for cell culture? Does it need purifying before use?
04 May 2016 1,826 2 View
Hi I am using Fibrinogen (10mg/ml) and Thrombin (1000U/ml) to form fibrin clots. I found that after getting good cross linking initially the clots dissolve to liquid if left in incubator...
04 May 2016 7,192 5 View
For Cell culture/bioreactor. Needs to be relatively quick setting
01 February 2016 9,357 1 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
I am using fibroblast growth factor, purchased in powder form. What suitable substance should be added to get it in a liquid form? Thanks
03 July 2024 9,866 2 View
"Dear Researchers, I am currently working on a Nanotechnology project and am exploring the process of drafting a patent to protect our innovations. Could anyone here share insights or tips on how...
02 July 2024 9,627 2 View
Give in detail with available literature and website links for submissions
27 June 2024 9,753 1 View
Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...
23 June 2024 4,902 3 View