Protocol for Inducing Necroptosis in Cell Culture ?

Hi I am looking for a protocol (with appropriate dilutions, concentrations, quantities) of inducing necroptosis in in vitro cell culture. I have found protocols using TNF alpha dosing and TAK1...

31 December 2019 5,798 3 View

How can one model/code for Momentum Capture in ABAQUS to capture waveforms at particular time points?

I am trying to model a waveform through different bars in Abaqus to mimic a SPHB. I wanted to add momentum capture so I can get values of the waveform at different time points. How does one do This?

03 April 2019 8,554 2 View

Anyone had any issues with fibrin gels disintegrating in OCT for immunostaining?

I use oCT to put my gels into in order to under go immunostaining. It appears the gels disintegrate both the longer they are in OCT and when they are frozen, leaving wafer thin samples. Does...

05 June 2016 3,193 0 View

Has anyone got an experience in using Bovine Thrombin from sigma?

- particularly this one http://www.sigmaaldrich.com/catalog/product/sigma/srp6556?lang=en®ion=US. Can it be used for cell culture? Does it need purifying before use?

04 May 2016 1,752 2 View

Problems with Bovine Fibrin Clots?

Hi I am using Fibrinogen (10mg/ml) and Thrombin (1000U/ml) to form fibrin clots. I found that after getting good cross linking initially the clots dissolve to liquid if left in incubator...

04 May 2016 7,107 5 View

Anyone have a good methodology for Agar Gel for adding on top of a cell culture dish (and that allows you to pour/pipette as a thin layer)?

01 February 2016 9,292 1 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Is nanotechnology part of materials science? Is it possible to obtain Pico technology and move from hypothesis to application?

Pico-scale This is three orders of magnitude smaller than a nanometer (and thus most nanotechnology) and two orders of magnitude smaller than most chemistry transformations and measurements. Pico...

18 February 2021 4,540 5 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View