Whether "lattice parameter" and "lattice constant" are refering to same constants like a,b,c in crystals?

In crystal structures, the lattice constants are defined as "a", "b" and "c". In few literature(s), the "lattice parameter" is used. Are they same?. Is there any difference?.

27 January 2019 7,088 4 View

What is the minimum cDNA template amount to use for BioTaq Polymerase reaction?

I am doing a PCR with BioTaq polymerase enzyme from Bioline. I am not seeing any positive bands on my gel. Does anyone know the minimum amount of template to use for this enzyme reaction?

15 October 2018 1,316 3 View

How useful is RIN value when you send out total RNA for miRNA sequencing?

I am extracting RNA from mouse tissue and would like to send the samples for sequencing, particularly looking for miRNAs in the samples. How useful and resourceful is the RIN value when the...

05 June 2018 3,300 3 View

Are there any specific microglial and astrocyte markers for primary cultures from postnatal mouse pups (P4-P7)?

Hi all. I am culturing primary astrocyte and microglia cells from postnatal mouse pups (P4-P7) and we are interested in looking at the gene expression specific for these cells types at this age....

06 March 2018 3,867 6 View

Can anyone share their experiences working with triple negative cell lines to screen for bioactivity?

Hi, I would like to hear suggestions about choosing cell lines for screening of small molecules. I understand that the common cell line used is MDAMB231. I also notice that use of MDAMB435 is...

09 February 2016 6,043 3 View

I am trying to run a flow cytometry experiment with MDAMB231 cells to do a cell cycle analysis. But why do I not see any cells when I run them?

06 August 2015 2,210 5 View

Is it possible to generate test cases from any IDE(Eclipse,Net-beans etc.,) after re-factoring ?

Software re-factoring has been done to improve the internal behavior of any code or application. After the re-factoring, some of the programs may report defects (say behavioral changes or...

07 November 2013 4,967 1 View

How are collagens separated from their source?

How to isolate type-I collagen from its crude source (chicken waste). Generally people use hydrolysis by acid or alkali but I am looking for biological extraction methods.

30 January 2013 6,561 1 View

RT-qPCR Analysis

RT -qPCR analysis- I am new to qPCR, I have done the PCR and got cut off values (Ct) and I plotted a std graph using the ct values of my diluted and amplified cDNA samples. I want to analyse my...

31 October 2012 2,017 10 View

RT activity assay

HI I am working on porcine tissue and looking for porcine endogenous retrovirus and testing its infectivity on HEK cell line by RT activity assay. It has been suggested that we should check the RT...

14 November 2011 149 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View