Hi Ashraf,

I do use a calibaration plate form our vendor (http://www.biotek.com/products/accessories/absorbance-test-plate/) But may be you can also use serial dilutions of a dye and lamda scans to verify your instrument.

Best Soenke

 Hi Soenke,

Thank you for your answer.  I wonder if you have the detailed procedure for the dye and lamda scans.

Dear Ashraf,

The procrdure might differ depending on you device. We are using a BioTek Synergy HT. The light for the absorbtion measurement is generated by a monochromator. So I can preform a lamda scan with i.e. every 5 nm a measurement (by software). The resulting curve of i.e. a dye like Trypan blue should be resamble the known spectra specialy with in the peak areas.

I you know take the dye and preform a serial dilution (in doubles) the linearity at the maximum absorbtion wave lenth, should be given at least for absorbens between 1-0.05.

If you have a calibration plate or Filter with a know optical density at wave lenth (xy) you can also use that for the verification.

Best Soenke

Dear Soenke,

Thanks a lot for your answer.

Dear Mr Weinert

Will this "calibration plate" have traceability certification along with it?

Dear Deepak,

at leat our calibration plate, came with a certificate which shows the manufactures data for each density filter and wave length and it comes with a date of expire or recalibration by the manufacture.

Did I answer your question?

kind regards Soenke

Can you name the manufacturer of this calibration plate?