How can I use the Cross Hi test for antibody evaluation?

More Serageldeen Sultan's questions See All

Can anybody give me with JCPDS card?

Answered.

02 July 2024 2,942 5 View

Can we use the mean and SD to describe continuous data then during comparison the authors use Mann-Whitney U-test ?

As I understand if you use the mean and SD for describing your data, this means it is normally distributed and parametric. If so, the test for variance should be simple T test.

04 April 2024 2,331 3 View

How we can find CI in RCTs, while we are only having incidences of adverse events.?

I am extracting data from RCTs for conducting a meta-analysis, and confused with the data extraction step

29 January 2024 501 0 View

Why several academic researchers are trying to develop research that may suits their statistical expertise ?

In fact, yes several researchers are thinking from the point of analysis only rather than trying to solve major research problems or questions. Therefore, the dilemma has also been shifted to...

05 August 2023 1,349 2 View

Is it ok for getting a high RMSD in MD simulation?

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24 July 2023 1,638 2 View

Hello Everyone, I am writing my bachelors thesis. I want a little help regarding the interpretation of moderation graph. Can you help me?

FinTech resistance is Dependent Variable Social Influenece is Independent Variable Technostress is Moderator

20 July 2023 4,653 1 View

Getting a high RMSD in protein-ligand MD simulation is ok ?

I've done a 50 ns protein-ligand MD simulation. Getting stable RMSD for the first 20 ns, but it increases afterwards, and at some point frame, it's getting very high. My question is, is my md...

19 June 2023 1,163 3 View

Did someone try a shredder from another brand than Qiagen?

Hi, I really need to replace the Qiashredder with a similar product. There is a number of companies that have competitive products. I would like to receive advice about them. Note: we work with...

10 June 2023 7,807 0 View

How to improve RNA extraction from mouth cells?

Hi, We will like to receive advice to improve RNA quality and quantity. After many trials and calibration, we get a protocol that gives better RNA quality and enough quantity for downstream...

10 June 2023 8,110 4 View

What parameter in protein-ligand MD simulation decides number of frames in the simulation output ?

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04 June 2023 9,418 4 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Geotechnical Engineering (Proceedings of the ICE) time review?

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10 August 2024 6,493 1 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

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07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How can I improve the quality of lamb milk replacer for machine feeding?

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06 August 2024 6,227 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Mohamed Elaish

Simply, it determine the relation ship between influenza viruses especially those sharing same HA protein. From that cross HI test, you determine the antigenic relatedness among different isolates expressed as an R-value based on the Archetti and Horsfall formula ( Archetti and Horsfall, 1950).

Serageldeen Sultan

Thanks Mohamed but;

How about its interpretation for other viruses than AIV, it is Useful?

Actually i don't know about other viruses like NDV. I can search the literature and inform you.