Serageldeen Sultan

5 Questions 12 Answers 0 Followers

Questions related from Serageldeen Sultan

Is it acceptable to do HI test using standard antigen of NDV after propagating it in the ECEs instead of buying new expensive one.

Usually we did HI test using NDV antigen (lentogenic strain) prepared by company. If we propagate this virus in ECEs it is approved to do HI test using the propagated strain instead of that...

13 June 2017 7,177 3 View

How can I use the Cross Hi test for antibody evaluation?

Is there any standardized protocol to do Hi test for evaluating the antibodies against vaccine virus strain and mismatched of these antibodies by wild viruses

03 December 2015 7,537 3 View

Non-specific PCR product?

I have used the primers VC-F cgg cgt ggc tgg ata cat tg and VC-R gtc aca ctt aaa tag tag cgt cc to amplify 389bp specific for V. cholera but every time a small band less than 100bp appears...

25 June 2013 5,569 12 View

What is the exact Tm of this primer pair?

I need help to determine the Tm of this primer pair Primer F 5' ATCCCAAGCCTTCTACC 3' Primer R 5' GCCACAGCAGTGTTTTCT' 3' I tried several annealing temp. 47 , 50, 51 and 52C but no...

29 October 2012 9,819 5 View

Estimation of the cellular immunity

Hello, I am going to do an experiment about the estimation of the cellular immunity, specially that convey by T cells in immunosupressive viral infection. So, I am wondering Is there a simple...

18 April 2012 7,784 5 View