6 Questions 17 Answers 0 Followers
Questions related from Serageldeen Sultan
Usually we did HI test using NDV antigen (lentogenic strain) prepared by company. If we propagate this virus in ECEs it is approved to do HI test using the propagated strain instead of that...
13 June 2017 7,253 3 View
Is there any standardized protocol to do Hi test for evaluating the antibodies against vaccine virus strain and mismatched of these antibodies by wild viruses
03 December 2015 7,545 3 View
I have used the primers VC-F cgg cgt ggc tgg ata cat tg and VC-R gtc aca ctt aaa tag tag cgt cc to amplify 389bp specific for V. cholera but every time a small band less than 100bp appears...
25 June 2013 5,582 12 View
Is there anybody who has done mean death time (MDT) in embryonated eggs. I am in urgent need of help because I did it using allantoic fluid 106 - 109 dilution but all the eggs died. So, do I have...
02 February 2013 9,254 5 View
I need help to determine the Tm of this primer pair Primer F 5' ATCCCAAGCCTTCTACC 3' Primer R 5' GCCACAGCAGTGTTTTCT' 3' I tried several annealing temp. 47 , 50, 51 and 52C but no...
29 October 2012 9,831 5 View
Hello, I am going to do an experiment about the estimation of the cellular immunity, specially that convey by T cells in immunosupressive viral infection. So, I am wondering Is there a simple...
18 April 2012 7,795 5 View
