I have prepared a stable cell line from HEK293 cells containing my gene of interest by using G418 as a selective agent. I want to transfect these cells with other genes. So do I need to add G418 everytime when I am working with a stable cell line.
May be I have not framed my question properly. I mean. I have generated stable cell line for 1 gene. Now I want to work using this cell line. So whenever I am transfecting this cell line for any experiment, do I need to add G418 in media everytime?
You can transfect your stable cell line trasiently with other gene but always use this cell line maintaining a low concentration of G418 in your culture medium to keep the selection