Pranjali Bhagwat

3 Questions 5 Answers 0 Followers

Questions related from Pranjali Bhagwat

Is it ok to use XhoI with NotI-HF for double digest? Please give suggestion.

I want to double digest my vector and insert with XhoI and NotI. I have normal XhoI and NotI HF. Is it ok to use this combination? What can be pros or cons. Appreciate your guidance. Thank you.

06 June 2017 5,908 0 View

How can I transfect a stable cell line produced by the antibiotic selection method?

I have prepared a stable cell line from HEK293 cells containing my gene of interest by using G418 as a selective agent. I want to transfect these cells with other genes. So do I need to add G418...

09 September 2014 4,747 3 View

How can I do mouse studies for a specific human receptor?

I am working with NKp30 a NK cell receptor which is not expressed in mice except the wild type Mus caroli. So how can I use mouse model for this study?

09 September 2014 413 1 View