A single chamber mediator free membrane-less MFC will be designed and fabricated. There will be two electrodes made up of stainless steel and copper etc. The anode and cathode will be connected by a multi-meter so as to measure the electrons transferred/ generated. Wastewater will be put into the chamber and experimentation will be carried out
MFC will be operated by varying process parameter such as By changing the type substrate /waste water i.e. using industrial waste water, and sewage water etc. By varying type of electrode i.e. copper and stainless steel etc.By changing the temperature and mixing speed.By changing the electrodes distance i.e. optimizing the distance between anode and cathode.
If you want to use urine, pH is not an operating parameter, it is fixed by human physiology around 7 (6.5 - 7.5). Anyway, if your final goal is to implement a MFC to treat urine, You should start reading the work of the Bristol research group. First of all, this:
X.A. Walter, I. Gajda, S. Forbes, J. Winfield, J. Greenman, I. Ieropoulos, Scaling-up of a novel, simplified MFC stack based on a self-stratifying urine column, Biotechnol. Biofuels. 9 (2016) 93. doi:10.1186/s13068-016-0504-3.