I want to see the effect of ethanol on a particular bacteria. The effect of it on the cellular physiology, metabolic changes, gene expression etc. What techniques are available to study this problem.
Viable cell count by exclusion method, total protein concentration, lactate dehydrogenase (LDH) activity and activation of apoptotic markers (P-H2AX, caspase-3 and PARP-1) are some methods to detect cytotoxic effect on trophoblast cells.
You can also refer to the following link to get details on the methods involved to detect the effects of ethanol on human HepaRG cell line:-
http://onlinelibrary.wiley.com/doi/10.1111/j.1530-0277.2010.01358.x/abstract
Metabolome analysis enables you to obtain a comprehensive data on the change in metabolic pathways. Mass spectrometry for metabolome analysis requires very few amount of proteins, so that you can easily submit samples for this analysis. The metabolic pathways are complicated and cross-talked (interact each other), so you have to know the comprehensive data.
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