Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How can we measure the reliability and validity of our survey questionnaire?

We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...

16 February 2021 678 3 View

Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

Do you consider it is important to vindicate Spanish as language, in scientific issues and publication of scientific articles?

03 February 2021 3,756 4 View

Do we need to keep looking for the fracture angle after the Puck's failure initiation is reached?

Greetings to everyone, Our team is currently working on the development of a VUMAT code in Abaqus for composite modelling using the Puck's failure theory as damage initiation criteria. Currently...

20 January 2021 6,367 1 View

Can my lysis buffer interfere with the quantification of DNA and RNA using Qubit2.0?

I am doing RNA and DNA extraction using the basic phenol / chloroform / isoamyl method to quantify RNA and DNA in aqueous phase, but first, I do a lysis with buffer (Tris-HCL 1M pH8; EDTA 0.5M pH8...

17 January 2021 1,453 3 View

How to design a reactive extraction process in Aspen Plus?

Hello, my task is it to create a specific extraction process. There is a mixture containing water, nitric acid and hydrochloric acid. It is known, that tributyl-phosphate is a solvent, which...

03 January 2021 4,163 3 View

Could speech recognition software be developed that DOESN'T convert sounds into waveforms? If not, what does it say about the semiotics of knowledge?

The standard approaches in speech recognition (and A.I. generally) rest on some very interesting assumptions that deserve to be carefully examined...

02 January 2021 9,363 11 View

Are there only winners in the world of exism?

BREXISM finally is over and they got a trade deal with europe now, but exism left the UK poorer according to the news Brexit is finally done. It will leave the UK...

23 December 2020 4,071 6 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

How reliable is estimation of copy numbers by absolute qPCR?

Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...

11 February 2021 8,421 13 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

How to estimation of reverse-transcription on tissue?

I would like to evaluate the amount of reverse transcription (cDNA) on a 20um fixed mouse brain section. I am using random primers with RevertAid H Minus M-MuLV (reverse transcriptase). and would...

08 February 2021 1,957 4 View

How could a transcriptional factor purified as inclusion bodies (by using urea) exhibit a ratio 260/280>1?

I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...

05 February 2021 2,530 3 View

RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...

05 February 2021 292 3 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View