How can I retrieve all nucleotide sequence of one bacteria collected from a certain country or geographical area?

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Pour la detection de l'apnee du sommeil en utilisant deep learning est ce que je peux passer par classification des stades de sommeil ?

pour la détection de l’apnée du sommeil en utilisant Deep Learning est ce que je peux passer par classification des stades de sommeil puis la détection de l'apnée et comment faire çà l'output du...

08 May 2023 9,198 2 View

Why do we often assign negative scores to gaps in sequence alignment?

Peace be upon all of you, Actually I have many questions: - why in sequence alignment are gaps expressed in a negative scores? - How can we count the number of possible alignments between two...

14 July 2022 4,857 0 View

Ferrite core inductor design ?

Dear all What is the optimal software package (free software is recommended) to design the ferrite core inductors, which can be used in the power converters, especially for the highly frequency...

23 September 2018 5,752 3 View

Concerning culture of Trichomonas vaginalis on MDCK culture media, what is the proper pH to suit both the cell media and the parasite?

I have searched for references and did not find any most references are talking about the ratio of the parasite to the media without mention of pH

20 April 2016 7,954 3 View

Why mosquitoes are able to transmit some viral disease but not all viruses?

How are some viruses able to multiply in mosquitoes while other just get digested? yellow fever virus for example but not HIV and if the HIV viruses get digested in mosquitoes why not the other...

23 February 2016 9,426 3 View

What are the dimensions of column used for protein purification?

Hello, i purify a different proteins from bacterial culture using gel filtration technique, i have 2 types of gel sephadex G20 and G100, i want to know: 1- the dimensions of the column i should ...

22 August 2015 8,341 7 View

FPGA for Power Electronics

What is the best low-cost FPGA for power electronics modulation and control?

01 January 1970 4,364 5 View

What is the appropriate medium for the isolation of hyper-thermophilic bacteria?

I want to isolate hyper thermophilic bacteria in general (not a specific bacterium) but i do not Know the most suitable medium; would u help me plz? thanks

01 January 1970 8,541 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What exactly is RAG-LLM doing? Isn’t it data engineering?

What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?

30 July 2024 7,376 3 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

I need the housing or real estate prices data since 1950 till date in India. Can anyone guide how can I get this data ?

I didn't find any data source for continuous time series since 1950. NHB Residex and RBI give data from 2013 and 2010 onwards. Please guide. Thanks !!!

27 July 2024 6,271 5 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View