I've read some protocols that they either add EDTA or dialyze the mixture against EDTA to remove the copper catalyst. What concentration of EDTA should be used? And which one is better, adding the EDTA then subsequently dialyzing against distilled water, or dialyzing against EDTA?
I have very little experience/success using EDTA, but look at Phophonics web page. I used a lot their products, just great and so easy to use: http://www.phosphonics.com/
If you can extract your compound in an organic solvent (DCM for instance) then use a EDTA solution (0,01M should be OK) to remove the catalyst. If your compound is water soluble and big enough you can then use dialysis against EDTA or with EDTA. There is also a resin called chelex that you can use you should avoid using it if your compound is charged it could be retained on the resin. http://www.bio-rad.com/webmaster/pdfs/9184_Chelex.PDF
Good luck!
Dear Maria,
I know this post is rather old, but here is an easy and effective method for Cu removal from water-soluble (and even Cu-coordinating) polymers that can otherwise be quite difficult to do.
Article Cu Elimination from Cu-Coordinating Macromolecules
Best of luck ;)- Badges
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