Maria Christine Tankeh Asuncion

11 Questions 17 Answers 0 Followers

Questions related from Maria Christine Tankeh Asuncion

Is it okay to conduct mammalian and yeast culture in the same facility ? Or does this run the risk of infecting the mammalian cell culture?

I was wondering if it's alright to use the same incubator and BSC for mammalian cells and yeast cells? Or is this a bad idea considering contamination? Thank you!

09 February 2018 1,433 3 View

Should I use NaOH or a buffer to change the pH of a protein solution?

One of the methodologies I would like to use state that they adjusted the pH of their protein solution to 9. However, they did not state how they did this. I am wondering if I should go ahead...

23 September 2015 1,401 6 View

What causes the smear at both edges of a traveling band in this agarose gel electrophoresis?

I'm running a 1% agarose gel electrophoresis, and I keep seeing "u-shaped" bands in my 1kb DNA ladder. The bands at the edges seem to be traveling slower and thus creating smears. What could be...

16 March 2015 1,863 7 View

How do you properly use alamar blue for a continuous monitoring over a period of time?

I would like to monitor cell metabolism over a period of several days (possibly beyond a week). Which of the two methods is correct? Or are both okay to do? a. Make separate wells for each...

10 November 2014 5,096 7 View

Do glass cover slips need to be coated with gelatin or fibronectin when dealing with mesenchymal stem cells?

I want to do immunocytochemistry with mesenchymal stem cells. I've been reading through different protocols and some mention having to coat their coverslips with gelatin or fibronectin whereas...

29 July 2014 4,248 2 View

What does "alternatively stain" mean in immunohistological procedures?

I'm trying to follow the methodology in one paper that describes the staining of cardiomyocytes with anti-connexin 43. After the procedure for staining, the paper states that "Samples we're...

19 July 2014 5,213 8 View

how do you do an evacuation-repressurization cycle for hexane displacement to measure porosimetry?

I'm trying to measure the porosity of some silk scaffolds I made. From some papers I read, they did a hexane displacement method to measure the porosity. Part of the methodology states that they...

08 July 2014 7,420 5 View

Why are the absorbance values using a traditional spectrophotometer and the nanodrop different?

We tried to compare the absorbance values of several copper sulfate standard solutions using Evolution 220 spectrophotometer (the traditional one), and the Nanodrop, but we we're surprised to find...

03 April 2014 9,472 3 View

How can I remove copper from a click reaction mixture using EDTA?

I've read some protocols that they either add EDTA or dialyze the mixture against EDTA to remove the copper catalyst. What concentration of EDTA should be used? And which one is better, adding...

02 March 2014 5,252 3 View

How to prepare borate buffer?

How do you prepare a borate buffer (100 mM borate, 150 mM NaCl, pH 9)? How would I know what system to use (e.g. boric acid-NaOH, sodium tetraborate-HCl, boric acid-sodium tetraborate, etc.) if it...

30 November 2013 7,675 11 View

What is the significance of enantiomeric purity for amino acids?

I'm planning to add functionalities to a protein molecule using certain amino acids in its backbone. I would just like to ask, why is it stated that "it is essential that amino acid building...

28 November 2013 9,720 8 View